- Highly Informative: includes a Degradation Index (ratio of short to long target quantity), IPC (Internal Positive Control) and Y-chromosome specific male target
- Highly Sensitive: limit of detection <1 picogram
- Highly Reproducible: large copy number of selected targets minimizes the effect of variation between individuals
- Easy to Use: compatible with commonly used real-time PCR instruments & software
Key Benefits:
- Improves first pass sample success rate
- Enables confident screening of negative samples
- Eliminates unnecessary sample retesting
- Enables selection of optimal downstream typing methods. i.e. autosomal STR, Y-STR or InnoTyper
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A Better System for Forensic Samples
Forensic samples are highly variable in DNA quantity and quality. Therefore, quantification is a critical step to ensure an appropriate amount of DNA, and downstream typing method, is used for downstream PCR amplification. Many current quantification systems do not fully account for quality differences in forensic samples and can underestimate the optimal amount of amplifiable DNA.
InnoQuant systems provide more comprehensive quality assessment by using two high copy number retrotransposable element targets: a short target (80 bp) for assessment of total DNA quantity, and a long target (207 bp) for better assessment of total amplifiable DNA. The ratio of short to long targets can be used to calculate the Degradation Index to assess the overall quality of a sample. The InnoQuant HY kit also includes a sensitive multi-copy Y-chromosome specific male target for additional information about a sample’s male DNA composition.

Highly Sensitive and Reproducible
InnoQuant systems are highly sensitive, reproducible and accurate due to the high copy nature of the assay targets. As shown in the figure below, the InnoQuant HY kit can consistently detect sub-picogram amounts of DNA. This enables you to confidently screen samples and avoid needless processing of negative samples.

In addition, the concentration of the pooled genomic male DNA used for the InnoQuant HY DNA Standard is calibrated against the NIST SRM 2372-A Human DNA Quantitation Standard to increase lot-to-lot consistency.
Adjust DNA Target to Improve Success Rates with Challenging Samples
When utilizing both the total DNA quantity and the Degradation Index, forensic analysts can better assess the optimal amount of DNA to amplify. Adjusting the amount of input DNA, on even moderately degraded samples, can greatly improve DNA profiles. As shown in the figure below, increasing the DNA input based on the InnoQuant long target results led to 130% more STR alleles being called.

Accurate Assessment of Male-Female Mixtures
With the inclusion of a sensitive multi-copy Y-chromosome specific male target, InnoQuant HY enables you to accurately determine male DNA quantity in challenging male-female mixtures (i.e. sexual assault samples), and more easily identify when sample processing should be stopped, modified, or if the sample should be preserved for alternative testing methods such as InnoTyper or Y-STR testing. As shown in the figure below, male DNA can be accurately detected in samples with large female DNA concentrations.

Improve Workflow and Eliminate Sample Retesting
A quantification method that provides more comprehensive information on sample quality can significantly improve the overall workflow in your laboratory. Better decisions can be made on which samples to amplify, the appropriate DNA target to amplify, and whether to concentrate or dilute a sample prior to amplification. This added information allows for a more streamlined workflow and minimizes unnecessary sample processing and retesting.
InnoQuant HY enables you to:
- Confidently screen negative samples
- Improve first pass success rate
- Eliminate unnecessary sample retesting
- Select optimal protocols and typing test kits
- Identify samples to preserve for alternative methods such as InnoTyper or Y-STR testing
COMPATIBLE WITH COMMONLY USED REAL-TIME PCR INSTRUMENTS & SOFTWARE
With multiple dye configuration options, the InnoQuant HY kit is fully compatible with the Applied Biosystems® 7500 Real-Time PCR System, the BioRad CFX Real-Time PCR System, and other instruments. Costly instrument or software upgrades are not required.



For research use only. Not for use in diagnostic procedures.
Protocols & Technical Resources
- InnoQuant HY User Guide for use with 7500 Real-Time PCR System
- InnoQuant Calibration Kit User Guide (for use with 7500 Real-Time PCR System)
- InnoQuant HY-R Basic Protocol for non-7500 Instruments
- SDS
Software Templates
- InnoQuant HID 1.x Run Template
- For other software templates, please contact us
Publications
- DNA and RNA profiling of excavated human remains with varying postmortem intervals. van den Berge, M. et al. Int J Legal Med (2016), pp 1471–1480
- Development and validation of InnoQuant, a sensitive human DNA quantitation and degradation assessment method for forensic samples using high copy number mobile elements Alu and SVA. G.M. Pineda et al., Forensic Sci. Int.: Genet. 13 (2014) 224–235
- Retrotransposable elements: Novel and sensitive DNA markers and their application in human identity. Forensic Sci. Int.: Genet. Suppl. Ser. 5 (2015) e627-e629.
- Comparison of Quantifiler® Trio and InnoQuant™ human DNA quantification kits for detection of DNA degradation in developed and aged fingerprints. Goecker, Zachary C. et al., Forensic Science International, Volume 263 (2016) 132 – 138
- The Width of a Human Hair: How New Technologies are Getting Results from Challenging Samples, Forensic Magazine, September 2015
Videos
Symposium Presentations & Posters
- Developmental validation of InnoQuant HY, a sensitive human and male DNA real-time PCR quantitation and degradation assessment system for forensic samples (ISHI 2016 Poster)
- Determining a stop point and optimal targeting values for downstream STR and Y-STR amplification using InnoQuant HY Quantitation and Quality Assessment System (ISHI 2016 Poster)
- InnoQuant as a Tool to Determine Profile Suitability and Improve Profile Success Rates for High Throughput Property Crime Specimens (ISHI 2015 Poster)
- Novel Methods to Obtain Nuclear DNA Profiles from Rootless Hair Shafts (ISHI 2015 Poster)
- Retrotransposable Elements: Novel and Sensitive DNA Markers and their Application in Human Identity (ISFG 2015 Poster)
- Development of New Technologies for DNA Analysis of Challenging Forensic Samples (AAFS 2015)
- Development of Novel DNA Analysis Methods to Improve Workflow Efficiency for Challenging Forensic Samples (WFF 2014 Oral Presentation)
- Improving Workflow Efficiency for Challenging Samples Utilizing New Technologies (ISHI 2014 Poster)
- Development of a Highly Sensitive Human and Male Quantification System for Assessing DNA Quality in Forensic Samples (AAFS 2014 Poster)
- Inter-Laboratory Testing of a Highly Sensitive Quantification System for Assessing DNA Quality in Forensic Samples(AAFS 2014 Poster)
- Development of a Highly Sensitive Quantification System for Assessing DNA Quality in Forensic Samples (ISHI 2013 Poster)
- Development of a Highly Sensitive Quantification System for Assessing DNA Quality in Forensic Samples (AAFS 2013 Oral Presentation)
Product | Size | Components | Catalog # | |
---|---|---|---|---|
InnoQuant HY Human and Male DNA Quantification & Degradation Assessment Kit | 250 reactions | DNA Standard – 75 µl @ 100 ng/µl Primer Mix – 2 tubes, 1.00 mL each Dilution Buffer A – 2 tubes, 1.80 mL each Agilent Technologies Brilliant Multiplex QPCR Master Mix – 2.5 mL ROX Reference Dye - 100 μL at 1 mM |
21108-250 | Request a Quote |
InnoQuant HY Human and Male DNA Quantification & Degradation Assessment Kit | 60 reactions | DNA Standard – 33 µl @ 100 ng/µl Primer Mix – 1 tube, 0.5 mL each Dilution Buffer A – 1 tube, 1.80 mL each Agilent Technologies Brilliant Multiplex QPCR Master Mix – 0.5 mL ROX Reference Dye - 100 μL at 1 mM |
21108-060 | Request a Quote |
InnoQuant HY-R Human and Male DNA Quantification & Degradation Assessment Kit (for non-7500 instruments) | 250 reactions | DNA Standard – 75 µl @ 100 ng/µl Primer Mix – 2 tubes, 1.00 mL each Dilution Buffer A – 2 tubes, 1.80 mL each Agilent Technologies Brilliant Multiplex QPCR Master Mix – 2.5 mL ROX Reference Dye - 100 μL at 1 mM |
21104-250 | Request a Quote |
InnoQuant HY-R Human and Male DNA Quantification & Degradation Assessment Kit (for non-7500 instruments) | 60 reactions | DNA Standard – 33 µl @ 100 ng/µl Primer Mix – 1 tube, 0.5 mL each Dilution Buffer A – 1 tube, 1.80 mL each Agilent Technologies Brilliant Multiplex QPCR Master Mix – 0.5 mL ROX Reference Dye - 100 μL at 1 mM |
21104-060 | Request a Quote |
InnoQuant Spectral Calibration Kit | 33 uL of each dye at 100 uM | Calibration standards for HEX, Cy3, Cy5, and TAMRA | 21105 | Request a Quote |
Storage Conditions: Upon receipt, store the kit at -20°C. After thawing, store all components at 2°C to 8°C.