InnoQuant® HY
InnoQuant® HY
- Fast Turnaround Time: Run time is under 45 min*
- Highly Informative and Reproducible: High Copy number targets and includes a Degradation Index (ratio of short to long target quantity), IPC (Internal Positive Control) and Y-chromosome specific male target
- Highly Sensitive: limit of detection <1 picogram
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InnoQuant HY Human and Male DNA Quantification & Degradation Assessment Kit (ROX Reference Dye supplied) (200 reactions)
DNA Standard, Primer Mix, Dilution Buffer A, Agilent Technologies Brilliant Multiplex QPCR Master Mix, ROX Reference Dye
InnoQuant HY Human and Male DNA Quantification & Degradation Assessment Kit (60 reactions)
DNA Standard, Primer Mix, Dilution Buffer A, Agilent Technologies Brilliant Multiplex QPCR Master Mix, ROX Reference Dye
InnoQuant HY-R Human and Male DNA Quantification & Degradation Assessment Kit (for non-7500/ instruments not running reference dye) (200 reactions)
DNA Standard, Primer Mix, Dilution Buffer A, Agilent Technologies Brilliant Multiplex QPCR Master Mix, ROX Reference Dye
InnoQuant HY-R Human and Male DNA Quantification & Degradation Assessment Kit (for non-7500 instruments) (60 reactions)
DNA Standard, Primer Mix, Dilution Buffer A, Agilent Technologies Brilliant Multiplex QPCR Master Mix, ROX Reference Dye
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Calibration standards for HEX, Cy3, Cy5, and TAMRA
Product Details
- A short Alu target (80 bp) for assessment of total DNA quantity
- A long SVA target (207 bp) for better assessment of total amplifiable DNA
- A multi-copy Y chromosome specific male target for identifying male samples even in sub-picogram level samples
- An Internal Positive Control (IPC) for inhibition detection.
- Confidently screen negative samples
- Improve first pass success rate
- Eliminate unnecessary sample retesting
- Select optimal protocols and typing test kits
- Identify samples to preserve for alternative methods such as InnoTyper or Y-STR testing
In addition, the concentration of the pooled genomic male DNA used for the InnoQuant HY DNA Standard is calibrated against the NIST SRM 2372-A Human DNA Quantitation Standard to increase lot-to-lot consistency.
Figure 1. A serial dilution of the NIST SRM 2372-A Human DNA Quantitation Standard was quantified in triplicate from neat solutions diluted to 0.078125 pg/µL, and mean concentrations compared to values listed in the SRM certificate.
In addition, the concentration of the pooled genomic male DNA used for the InnoQuant HY DNA Standard is calibrated against the NIST SRM 2372-A Human DNA Quantitation Standard to increase lot-to-lot consistency.
In studies with real casework samples, the InnoQuant long target quant data correlated with downstream STR profile success significantly better than other quantification targets. (For complete study details, see here.)
Figure 2. Degradation study results. Peak height averages of Yfiler alleles detected above 50 RFU with percentage of alleles recovered as sonication time and Degradation Index increases. The data indicates the long target of InnoQuant HY can be effectively used to target Yfiler amplifications to obtain a more complete profile.
Figure 3. Mean quantification results from a series of samples run in triplicate that were prepared with mixtures of human male (NIST SRM 2372-A) and human female (NIST SRM 2372-B) DNA. The male DNA was kept at a constant level of 10 pg/µL while the female DNA was added at increasing levels to create the male-to-female ratio range from 1:1 to 1:8000. The InnoQuant HY kit was able to detect male DNA up to the highest ratio of 1:8000 tested. Additional studies conducted by a third party laboratory have demonstrated the kit’s ability to detect as little as 1 pg/µL of male DNA in a male-to-female ratio of approximately 1:50,000 (data not shown).
Figure 4. Dye configurations for the InnoQuant HY and InnoQuant HY-R kits. The InnoQuant HY kit is recommended for use with the Applied Biosystems® 7500 Real-Time PCR System, while the InnoQuant HY-R kit is recommended for other RT-PCR instrument platforms that do not require the use of a reference dye.
Figure 5. Amplification plots of the DNA Standards and NTC for short (A), long (B), Y (C) and IPC (D) targets.
Resources
- InnoQuant HID 1.x Run Template
- For other software templates, please contact us
- DNA and RNA profiling of excavated human remains with varying postmortem intervals. van den Berge, M. et al. Int J Legal Med (2016), pp 1471–1480
- Development and validation of InnoQuant, a sensitive human DNA quantitation and degradation assessment method for forensic samples using high copy number mobile elements Alu and SVA. G.M. Pineda et al., Forensic Sci. Int.: Genet. 13 (2014) 224–235
- Retrotransposable elements: Novel and sensitive DNA markers and their application in human identity. Forensic Sci. Int.: Genet. Suppl. Ser. 5 (2015) e627-e629.
- Comparison of Quantifiler® Trio and InnoQuant™ human DNA quantification kits for detection of DNA degradation in developed and aged fingerprints. Goecker, Zachary C. et al., Forensic Science International, Volume 263 (2016) 132 – 138
- The Width of a Human Hair: How New Technologies are Getting Results from Challenging Samples, Forensic Magazine, September 2015
- Developmental validation of InnoQuant HY, a sensitive human and male DNA real-time PCR quantitation and degradation assessment system for forensic samples (ISHI 2016 Poster)
- Determining a stop point and optimal targeting values for downstream STR and Y-STR amplification using InnoQuant HY Quantitation and Quality Assessment System (ISHI 2016 Poster)
- InnoQuant as a Tool to Determine Profile Suitability and Improve Profile Success Rates for High Throughput Property Crime Specimens (ISHI 2015 Poster)
- Novel Methods to Obtain Nuclear DNA Profiles from Rootless Hair Shafts (ISHI 2015 Poster)
- Retrotransposable Elements: Novel and Sensitive DNA Markers and their Application in Human Identity (ISFG 2015 Poster)
- Development of New Technologies for DNA Analysis of Challenging Forensic Samples (AAFS 2015)
- Development of Novel DNA Analysis Methods to Improve Workflow Efficiency for Challenging Forensic Samples (WFF 2014 Oral Presentation)
- Improving Workflow Efficiency for Challenging Samples Utilizing New Technologies (ISHI 2014 Poster)
- Development of a Highly Sensitive Human and Male Quantification System for Assessing DNA Quality in Forensic Samples (AAFS 2014 Poster)
- Inter-Laboratory Testing of a Highly Sensitive Quantification System for Assessing DNA Quality in Forensic Samples(AAFS 2014 Poster)
- Development of a Highly Sensitive Quantification System for Assessing DNA Quality in Forensic Samples (ISHI 2013 Poster)
- Development of a Highly Sensitive Quantification System for Assessing DNA Quality in Forensic Samples (AAFS 2013 Oral Presentation)