InnoQuant®

InnoQuant Kit

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InnoQuant® Kit – highly sensitive and robust assessment of human DNA quantity, quality and integrity.

  • Highly Informative: includes a Degradation Index (ratio of short to long target quantity) and IPC (Internal Positive Control) for comprehensive DNA quality assessment
  • Highly Sensitive: limit of detection
  • Highly Reproducible: the large copy number of selected targets (>1000 copies/genome) minimizes effect of variation between individuals
  • Easy to Use: compatible with commonly used real-time PCR instruments & software

Key Benefits:

  • Improves first pass sample success rate
  • Enables confident screening of negative samples
  • Eliminates unnecessary sample retesting
  • Enables selection of optimal downstream typing methods and protocols
InnoQuant is a highly sensitive real-time qPCR assay for robust simultaneous assessment of human DNA quantity, quality and integrity. The use of high copy number retrotransposable element targets provides excellent sensitivity and reproducibility.

A Better System for Forensic Samples

Forensic samples are highly variable in DNA quantity and quality. Therefore, quantification is a critical step to ensure an appropriate amount of DNA, and downstream typing method, is used for downstream PCR amplification. Many current quantification systems do not fully account for quality differences in forensic samples and can underestimate the optimal amount of amplifiable DNA.

InnoQuant systems provide more comprehensive quality assessment by using two high copy number retrotransposable element targets: a short target (80 bp) for assessment of total DNA quantity, and a long target (207 bp) for better assessment of total amplifiable DNA. The ratio of short to long targets can be used to calculate the Degradation Index to assess the overall quality of a sample.

Degradation Index vs Peak RFU
Figure 1. The InnoQuant degradation index (DI = short quantity value / long quantity value) accurately assesses degradation in a forensic sample. As the DI increases, the peak height (RFU) of STR alleles in downstream profiling decreases.

Highly Sensitive and Reproducible

The InnoQuant system is highly sensitive due to the multi-copy nature of its targets, and highly reproducible and accurate due to their high copy number (>1000 copies per genome). This system consistently detects sub-picogram amounts of DNA, enabling you to confidently screen samples and avoid needless processing of negative samples. (For complete sensitivity study details, see here.)

In addition, the concentration of the genomic male DNA used for the InnoQuant DNA Standard is calibrated against the NIST SRM 2372 Human DNA Quantitation Standard to increase lot-to-lot consistency.

Adjust DNA Target to Improve Success Rates with Challenging Samples

When utilizing both the total DNA quantity and the Degradation Index, forensic analysts can better assess the optimal amount of DNA to amplify. Adjusting the amount of input DNA, on even moderately degraded samples, can greatly improve DNA profiles. As shown in the figure below, increasing the DNA input based on the InnoQuant long target results led to 130% more STR alleles being called.

Using InnoQuant Can Improve Profile Success
Figure 2. Increasing the DNA input based on the Degradation Index and the InnoQuant long target results led to 130% more alleles being called. (Allele detection measured using Identifiler® Plus PCR Amplification Kit and Applied Biosystems 3130 Genetic Analyzer.) Use of both the Degradation Index and the quantity values can improve STR allele recovery and reliably predict downstream STR success.

In studies with real casework samples, the InnoQuant long target quant data correlated with downstream STR profile success significantly better than other quantification targets. (For complete study details, see here.)

Improve Workflow and Eliminate Sample Retesting

A quantification method that provides more comprehensive information on sample quality can significantly improve the overall workflow in your laboratory. Better decisions can be made on which samples to amplify, the appropriate DNA target to amplify, and whether to concentrate or dilute a sample prior to amplification. This added information allows for a more streamlined workflow and minimizes unnecessary sample processing and retesting.

InnoQuant enables you to:

  • Confidently screen negative samples
  • Improve first pass success rates
  • Eliminate unnecessary retesting
  • Select optimal protocols and typing test kits
  • Identify samples to preserve for alternative methods such as InnoTyper or mini-STR testing

COMPATIBLE WITH COMMONLY USED REAL-TIME PCR INSTRUMENTS & SOFTWARE

With multiple dye configuration options, the InnoQuant kit is fully compatible with the Applied Biosystems® 7500 Real-Time PCR System, the BioRad CFX Real-Time PCR System, and other instruments. Costly instrument or software upgrades are not required.

Dye Configurations for H-dye Kits
Figure 3. Dye configurations for the InnoQuant and InnoQuant H-dye kits. The multiple options enable increased compatibility with various commonly used RT-PCR instrument platforms.
InnoQuant Amplification Plots
Figure 4. Amplification plots of the DNA Standards and NTC for short (A), long (B), and IPC (C) targets.
Standard Curve
Figure 5. Standard curves of the short and long targets using DNA standard dilutions ranging in concentration from 20 ng/µL to 0.005 ng/µL.

For research use only. Not for use in diagnostic procedures.

Protocols & Technical Resources

Software Templates

Videos

Publications

Symposium Presentations & Posters

Product Size Components Catalog #
InnoQuant Human DNA Quantification & Degradation Assessment Kit 250 reactions DNA Standard – 75 µl @ 100ng/µl
Primer Mix – 2 tubes, 1.00 mL each
Dilution Buffer A – 2 tubes, 1.80 mL each
Agilent Technologies Brilliant Multiplex QPCR Master Mix – 2.5 mL
ROX Reference Dye - 100 μL at 1 mM
21107-250 Request a Quote
InnoQuant Human DNA Quantification & Degradation Assessment Kit 60 reactions DNA Standard – 33 µl @ 100 ng/µl
Primer Mix – 1 tube, 0.5 mL each
Dilution Buffer A – 1 tube, 1.80 mL each
Agilent Technologies Brilliant Multiplex QPCR Master Mix – 0.5 mL
ROX Reference Dye - 100 μL at 1 mM
21107-060 Request a Quote
InnoQuant H-dye DNA Quantification & Degradation Assessment Kit 250 reactions DNA Standard – 75 µl @ 100 ng/µl
Primer Mix – 2 tubes, 1.00 mL each
Dilution Buffer A – 2 tubes, 1.80 mL each
Agilent Technologies Brilliant Multiplex QPCR Master Mix – 2.5 mL
ROX Reference Dye - 100 μL at 1 mM
21106-250 Request a Quote
InnoQuant H-dye DNA Quantification & Degradation Assessment Kit 60 reactions DNA Standard – 33 µl @ 100 ng/µl
Primer Mix – 1 tube, 0.5 mL each
Dilution Buffer A – 1 tube, 1.80 mL each
Agilent Technologies Brilliant Multiplex QPCR Master Mix – 0.5 mL
ROX Reference Dye - 100 μL at 1 mM
21106-060 Request a Quote
InnoQuant Spectral Calibration Kit 33 uL of each dye at 100 uM Calibration standards for HEX, Cy3, Cy5, and TAMRA 21105 Request a Quote

 

Storage Conditions: Upon receipt, store the kit at -20°C. After thawing, store all components at 2°C to 8°C.