InnoTyper® 21

InnoTyper® 21

Analyze Less. Solve More.

A small amplicon (~60-125 bp) DNA typing system for challenging forensic samples that is compatible with currently used Capillary Electrophoresis (CE) instrument platforms.

  • Obtain DNA profiles from Rootless Hairs
  • Achieve informative results with degraded/low level samples
  • High sensitivity –obtaining profiles with as little as 15 – 30 picograms total DNA*
  • Significantly more discriminating and less labor-intensive than mitochondrial DNA sequencing
  • Compatible with DNA•VIEW DNA Analysis Software

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Catalog #21109-100
InnoTyper 21 Human DNA Typing Kit (100 reactions)
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DNA Control, Primer Mix, Master Mix, IGT DNA Polymerase, Allelic Ladder
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Catalog #21109S-80

IGT ILS-155 Size Standard (80 µL)

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Catalog #21109M-35
IGT 5 Dye Matrix Standard for 3500 Series Genetic Analyzers (80 µL)
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Catalog #21109M-31
IGT 5 Dye Matrix Standard for 3130 Series Genetic Analyzers (80 µL)
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Product Details

Overview
InnoTyper® 21 is a small amplicon DNA typing system containing 20 Insertion/Null (INNUL) markers and Amelogenin that is fully compatible with commonly used and validated PCR and capillary electrophoresis (CE) platforms.
With an amplicon size range of only ~60-125bp, this kit provides high sensitivity, high tolerance to degradation and inhibition, and a high discrimination power (approximately 1 in 100 million). It can obtain informative results from extremely degraded and/or low-level single-source forensic samples, including bone fragments or single hair shafts that typically yield minimal results with other systems.
The INNUL markers in this kit are neutral genetic loci with stable polymorphisms that are not deleted and identical by descent only, providing valuable utility for difficult kinship, missing person and mass disaster victim identification cases. INNUL markers have been well characterized but previous typing attempts have been unsuccessful due to the large size of the insertion.
InnoTyper 21 uses a novel mini-primer design to control the size of the resultant insertion or null alleles. As shown in Figure 1, the amplicon size range of InnoTyper 21 markers is considerable smaller when compared to commonly used STR kits. This gives InnoTyper 21 an advantage to type forensic samples that have been previously unsuccessful, even with mini-STR analysis.
InnoTyperFig1
Figure 1. Comparison of amplicon sizes (base pairs) between widely available forensic typing kits.

With the ability to obtain informative results from heavily degraded samples with as little as 15 – 30 picograms total DNA*, InnoTyper 21 provides a discriminating alternative to mitochondrial DNA (mtDNA) sequencing, which is currently the genotyping standard for degraded samples such as old human remains and hair shafts. InnoTyper 21 is significantly more discriminating and less labor-intensive than mtDNA sequencing, and does not require a specialized workflow.

Discrimation Power for Fragmented Samples

InnoTyper 21 kit proved to be more discriminating than traditional STR multiplexes for DNA fragments less than 150 base pairs in size (For complete study details, see here.)

InnoTyperFig2
Figure 2. 
Obtain Profiles from Difficult Sample Types
With IT21’s high copy, small amplicon sizes, the ability to obtain profiles from rootless hair shafts and degraded bone samples is now possible.
InnoTyperFig3
Figure 3.  Rootless Hair shafts
InnoTyperFig4
Figure 4.  InnoTyper 21 DNA profile of a 2-cm rootless hair shaft.
Bone Fragments

Studies have also demonstrated the utility of InnoTyper 21 for highly compromised human remains, i.e. bone fragments, that have failed to yield informative results using other methods, as shown in Figure 4. (For complete study details, see here.)

*Depends on the DNA quantification system used to measure DNA input amount. For research use only. Not for use in diagnostic procedures

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