Analyze Less. Solve More.
- Can obtain informative results from extremely degraded and/or low-level forensic samples, including bone fragments and rootless hair shafts
- High sensitivity – informative profiles can be obtained with as little as 15 - 30 picograms total DNA*
- High tolerance to degradation and inhibition
- Significantly more discriminating and less labor-intensive than mitochondrial DNA sequencing
- Fully compatible with commonly used and validated instrument platforms
- Bi-allelic system simplifies data analysis
- Stable polymorphisms that are not deleted and identical by descent only, providing valuable utility for difficult kinship, missing person and mass disaster victim identification cases
This product is NOW AVAILABLE! Click here to try in your lab.
With an amplicon size range of only ~60-125bp, this kit provides high sensitivity, high tolerance to degradation and inhibition, and a high discrimination power (approximately 1 in 100 million). It can obtain informative results from extremely degraded and/or low-level single-source forensic samples, including bone fragments or single hair shafts that typically yield minimal results with other systems.
The RIP markers in this kit are neutral genetic loci with stable polymorphisms that are not deleted and identical by descent only, providing valuable utility for difficult kinship, missing person and mass disaster victim identification cases. RIP markers have been well characterized but previous typing attempts have been unsuccessful due to the large size of the insertion.
InnoTyper 21 uses a novel mini-primer design to control the size of the resultant insertion or null alleles. As shown in Figure 1, the amplicon size range of InnoTyper 21 markers is considerable smaller when compared to commonly used STR kits. This gives InnoTyper 21 an advantage to type forensic samples that have been previously unsuccessful, even with mini-STR analysis.
With the ability to obtain informative results from heavily degraded samples with as little as 15 - 30 picograms total DNA*, InnoTyper 21 provides a discriminating alternative to mitochondrial DNA (mtDNA) sequencing, which is currently the genotyping standard for degraded samples such as old human remains and hair shafts. InnoTyper 21 is significantly more discriminating and less labor-intensive than mtDNA sequencing, and does not require a specialized workflow.
In studies conducted by the NIST (National Institute of Standards and Technology) Applied Genetics Group, the InnoTyper 21 kit proved to be more discriminating than traditional STR multiplexes for DNA fragments less than 150 base pairs in size, as shown in Figure 2. (For complete study details, see here.)
Studies with hair shafts have demonstrated that InnoTyper 21 may be used to successfully type these rarely utilized samples, as shown in Figure 3. (For complete study details, see here.)
Studies have also demonstrated the utility of InnoTyper 21 for highly compromised human remains, i.e. bone fragments, that have failed to yield informative results using other methods, as shown in Figure 4. (For complete study details, see here.)
*Depends on the DNA quantification system used to measure DNA input amount.
For research use only. Not for use in diagnostic procedures.
PROTOCOLS & TECHNICAL RESOURCES
SOFTWARE ANALYSIS FILES
- InnoTyper 21 Panels – for use with POP4 and GeneMapper® ID-X Software
- InnoTyper 21 Bins – for use with POP4 and GeneMapper® ID-X Software
- InnoTyper 21 Panels – for use with POP4 and GeneMapper® ID Software
- InnoTyper 21 Bins – for use with POP4 and GeneMapper® ID Software
- DNA and RNA profiling of excavated human remains with varying postmortem intervals. van den Berge, M. et al. Int J Legal Med (2016), pp 1471–1480
- Retrotransposable elements: Novel and sensitive DNA markers and their application in human identity. Forensic Sci. Int.: Genet. Suppl. Ser. 5 (2015) e627-e629.
- The Width of a Human Hair: How New Technologies are Getting Results from Challenging Samples, Forensic Magazine, September 2015
- INNULs: A novel design amplification strategy for retrotransposable elements for studying population variation. Human heredity, 74(1) (2012) 27-35.
Symposium Presentations & Posters
- Developmental validation of InnoTyper 21, a nuclear DNA typing system based on retrotransposable element polymorphisms for degraded forensic samples (ISHI 2016 Poster)
- Validation of the InnoQuant and InnoTyper 21 Kits for Use on Forensic Samples (DNA Solutions ISHI 2016 Poster)
- Obtaining Probative Results from Human Remains Recovered from Conflict Regions Using Multiple Systems in Parallel (DNA Solutions ISHI 2016 Poster)
- Use of InnoTyper 21 with Highly Degraded Missing Persons Samples (UNTHSC Webinar Presentation, June 29, 2016)
- Examination of 20 Retrotransposable Polymorphic Insertion/Null (INNUL) markers for their utility in kinship testing (NIST AAFS 2016 Poster)
- Working With Challenging Samples: An Independent Assessment of the Relative Performance of the Promega Fusion™ and InnoGenomics InnoTyper 21 Kit With Probative Samples (DNA Solutions AAFS 2016 Poster)
- Utility of InnoTyper 21 in Analysis of Degraded Human DNA Recovered from Maggot Crop Contents (VCU AAFS 2016 Poster)
- Novel Methods to Obtain Nuclear DNA Profiles from Rootless Hair Shafts (ISHI 2015 Poster)
- Retrotransposable Elements: Novel and Sensitive DNA Markers and their Application in Human Identity (ISFG 2015 Poster)
- Utility of a Novel and Sensitive DNA Multiplex for Highly Degraded Missing Persons Samples (UNTHSC AAFS 2015 Oral Presentation)
- Development of New Technologies for DNA Analysis of Challenging Forensic Samples (AAFS 2015 Workshop Presentation)
- Development of Novel DNA Analysis Methods to Improve Workflow Efficiency for Challenging Forensic Samples (WFF 2014 Oral Presentation)
- Improving Workflow Efficiency for Challenging Samples Utilizing New Technologies (ISHI 2014 Poster Presentation)
- InnoTyper: A Novel Approach to Genotyping Degraded DNA Samples Utilizing LINEs and SINEs (UNTHSC AFDAA 2014 Oral Presentation)
- Development of a Novel and Sensitive DNA Analysis Multiplex Based on INNUL Markers for Highly Degraded Forensic DNA Samples (AAFS 2014)
- Development of a Novel Multiplexed DNA Analysis System for Highly Degraded DNA Samples (ISHI 2013)
|InnoTyper 21 Human DNA Typing Kit||100 reactions||DNA Control – 100 μL @ 0.12ng/μL
Primer Mix – 385 μL
Master Mix – 550 μL
IGT DNA Polymerase – 55 μL
Allelic Ladder – 30 μL
|21109-100||Request a Quote|
|InnoTyper 21 Human DNA Typing Kit||50 reactions||DNA Control – 50 μL @ 0.12ng/μL
Primer Mix – 193 μL
Master Mix – 275 μL
IGT DNA Polymerase – 28 μL
Allelic Ladder – 15 μL
|21109-50||Request a Quote|
|IGT ILS-155 Size Standard||50 µL||ILS-155 internal lane standard (50 µL)||21109ILS||Request a Quote|
|IGT 5 Dye Matrix Standard for 3500 Series Genetic Analyzers||80 µL||IGT 5 Dye Matrix Standard for 3500 Series Genetic Analyzers (80 µL)||21109M-35||Request a Quote|
|IGT 5 Dye Matrix Standard for 3130 Series Genetic Analyzers||80 µL||IGT 5 Dye Matrix Standard for 3130 Series Genetic Analyzers (80 µL)||21109M-31||Request a Quote|
Storage Conditions: Upon receipt, store the kit at -20°C. After initial use, store the kit at +4°C.