InnoTyper® 21

InnoTyper 21 Kit

Analyze Less. Solve More.

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InnoTyper® 21 Kit - a small amplicon (~60-125 bp) DNA typing system for challenging forensic samples that is compatible with currently used instrument platforms.

  • Can obtain informative results from extremely degraded and/or low-level forensic samples, including bone fragments and rootless hair shafts
  • High sensitivity – informative profiles can be obtained with as little as 15 - 30 picograms total DNA*
  • High tolerance to degradation and inhibition
  • Significantly more discriminating and less labor-intensive than mitochondrial DNA sequencing
  • Fully compatible with commonly used and validated instrument platforms
  • Bi-allelic system simplifies data analysis
  • Stable polymorphisms that are not deleted and identical by descent only, providing valuable utility for difficult kinship, missing person and mass disaster victim identification cases

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InnoTyper® 21 is a small amplicon DNA typing system containing 20 Retrotransposable Insertion Polymorphism (RIP) markers and Amelogenin that is fully compatible with commonly used and validated PCR and capillary electrophoresis (CE) platforms.

With an amplicon size range of only ~60-125bp, this kit provides high sensitivity, high tolerance to degradation and inhibition, and a high discrimination power (approximately 1 in 100 million). It can obtain informative results from extremely degraded and/or low-level single-source forensic samples, including bone fragments or single hair shafts that typically yield minimal results with other systems.

The RIP markers in this kit are neutral genetic loci with stable polymorphisms that are not deleted and identical by descent only, providing valuable utility for difficult kinship, missing person and mass disaster victim identification cases. RIP markers have been well characterized but previous typing attempts have been unsuccessful due to the large size of the insertion.

InnoTyper 21 uses a novel mini-primer design to control the size of the resultant insertion or null alleles. As shown in Figure 1, the amplicon size range of InnoTyper 21 markers is considerable smaller when compared to commonly used STR kits. This gives InnoTyper 21 an advantage to type forensic samples that have been previously unsuccessful, even with mini-STR analysis.

Technology Comparison of InnoTyper 21
Figure 1. Comparison of amplicon sizes (base pairs) between widely available forensic typing kits. InnoTyper 21 contains the smallest amplicon size range, providing an advantage for extremely challenging forensic samples that typically yield minimal results with other systems.

With the ability to obtain informative results from heavily degraded samples with as little as 15 - 30 picograms total DNA*, InnoTyper 21 provides a discriminating alternative to mitochondrial DNA (mtDNA) sequencing, which is currently the genotyping standard for degraded samples such as old human remains and hair shafts. InnoTyper 21 is significantly more discriminating and less labor-intensive than mtDNA sequencing, and does not require a specialized workflow.

In studies conducted by the NIST (National Institute of Standards and Technology) Applied Genetics Group, the InnoTyper 21 kit proved to be more discriminating than traditional STR multiplexes for DNA fragments less than 150 base pairs in size, as shown in Figure 2. (For complete study details, see here.)

RMP_DegradedFragments
Figure 2. Fragment size versus Random Match Probability for three commercial kits (InnoTyper 21, GlobalFiler®, and MiniFiler®). A single sample was degraded using a Covaris S2 Sonicator into 75, 100, 150, 200, and 250 bp fragments. Three replicates were run for each commercial kit. (Data courtesy of the NIST Applied Genetics Group.)

Studies with hair shafts have demonstrated that InnoTyper 21 may be used to successfully type these rarely utilized samples, as shown in Figure 3. (For complete study details, see here.)

InnoTyper 21 DNA profile from 2 cm rootless hair
Figure 3. InnoTyper 21 DNA profile of a 2-cm rootless hair shaft run on an Applied Biosystems© 3130 Genetic Analyzer. A significant percentage of hair shafts processed with InnoTyper 21 can provide usable genotype data with statistical power superior to mtDNA, enabling forensic laboratories to process hair shafts using standard instrument platforms currently in place.

Studies have also demonstrated the utility of InnoTyper 21 for highly compromised human remains, i.e. bone fragments, that have failed to yield informative results using other methods, as shown in Figure 4. (For complete study details, see here.)

InnoTyper 21 DNA profile of degraded human remains
Figure 4. InnoTyper 21 DNA profile of human remains which previously yielded no interpretable results with STR and mitochondrial DNA profiling methods. (Data courtesy of the UNTHSC Center for Human Identification.)

*Depends on the DNA quantification system used to measure DNA input amount.

For research use only. Not for use in diagnostic procedures.

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Product Size Components Catalog #
InnoTyper 21 Human DNA Typing Kit 100 reactions DNA Control – 100 μL @ 0.12ng/μL
Primer Mix – 385 μL
Master Mix – 550 μL
IGT DNA Polymerase – 55 μL
Allelic Ladder – 30 μL
21109-100 Request a Quote
InnoTyper 21 Human DNA Typing Kit 50 reactions DNA Control – 50 μL @ 0.12ng/μL
Primer Mix – 193 μL
Master Mix – 275 μL
IGT DNA Polymerase – 28 μL
Allelic Ladder – 15 μL
21109-50 Request a Quote
IGT ILS-155 Size Standard 50 µL ILS-155 internal lane standard (50 µL) 21109ILS Request a Quote
IGT 5 Dye Matrix Standard for 3500 Series Genetic Analyzers 80 µL IGT 5 Dye Matrix Standard for 3500 Series Genetic Analyzers (80 µL) 21109M-35 Request a Quote
IGT 5 Dye Matrix Standard for 3130 Series Genetic Analyzers 80 µL IGT 5 Dye Matrix Standard for 3130 Series Genetic Analyzers (80 µL) 21109M-31 Request a Quote

 

Storage Conditions: Upon receipt, store the kit at -20°C. After initial use, store the kit at +4°C.